Re: doxorubicin and viability dyes
Mario Roederer (Roederer@Darwin.Stanford.EDU)
Thu, 14 Aug 1997 09:24:47 -0800
> Hopefully, without giving the impression of being too
> picky, I need a viability dye that will:
> (a) not overlap with doxorubicin's endogenous red
> fluorescence
> (b) not employ the mechanism of DNA intercalation so it
> doesn't interfere with the cell damaging action
> of doxorubicin
How about using PE-Annexin V, and then you can use FDA (fluorescein di-acetate)
as a viability dye (it stains LIVE cells rather than dead cells)? I believe
that PharMingen now has PE-Annexin V; FDA can be purchased cheaply from a number
of sources.
mr