Have you ever experienced TdT positive AML M3?
The patient was in his 30s and the bone marrow
was clouded with typical M3 promyelocytes.
They expressed surface antigen of CD13+and CD33+ without
HLA-DR.
All lymphoid lineage markers were negative.
Our protocol was as follows;
1. Mononuclar cell separation with Ficoll-Hypaque
2. Wash with 1%BSA-PBS once
3. Permeabilization with FACS Brand Lysing Solution
4. Wash with 1%BSA-PBS once
5. Incubation with anti-TdT MAb
6. Wash with 1%BSA-PBS once
7. Data aqusition and analysis with FACSort and HP340
computer system
Autofluorescence without anti-TdT MAb was used as negative
control and MOLT-3 cell line was used as positive control.
About over 90 percent of promyelocytes were nuclear TdT
positive.
What's wrong or was it real?
Chang-Seok Ki, MD
Dept. of Clinical Pathology
Samsung Medical Center