I'm a newbie here on this board--though, not necessarily a newbie in flow
cytometry. I have about 10 years experience in flow--but, I have also
been working in other areas of the lab at the same time, i.e.,
hematology, coagulation, urinalysis, and special hematology testing. So,
I guess I'm more a jack of all trades, rather than a master of this one.
That is why I'm asking such a basic question.
When I worked at the Johns Hopkins Oncology Center, and did flow
cytometric analyses of leukemias, we used leukemia cell lines (KG1a,
Daudi, and MOLT3 cells) as positive controls for our leukemia antibodies.
The cells were supplied to us by one of the oncology research labs.
Now I work at a community hospital that has a flow cytometry lab, and I
no longer have access to these cell lines. Does anybody know if there
are stabilized leukemia cell lines that are sold commercially? As you
can see, they don't let me out of my cage too
often.......................
Thanks very much in advance for any information you can give me.
Going with the flow,
Terry Fecik
Sinai Hospital of Baltimore