<<Subject: Lymph Node Dissociation Methods
From: Bill Justice <bjustice@kumc.edu> at smtplink-nedh
Date: 7/29/97 06:35 AM
Hello
I am asking this question on behalf of another flow cytometry operator at our
institution.
This is a very basic question. When processing lymph node for L/L panels,
what
are other labs doing to mechanically process the node?
Tissue Homogenizers?
scissors and scalpels?
pushing through wire mesh?
What?
What are the pitfall and potential problems with difference techniques?
Thanks.
Bill Justice
Flow Cytometry/PC Support
KUMC Clinical Labs
>>
when i used to work with monkey tissues in a previous lab incarnation, we
used the top ends of syringe plungers to mash up whole or pieces of lymph
nodes and other organs in petri dishes. These were the10ml size with the
ridged tops, not smooth. we then put the whole mush thru falcon 100um mesh
filters for 50 ml tubes, rinsing it through with additional media. it worked
well for flow analysis and culture.
-brian white
human genome sciences
rockville, md
brian_white@hgsi.com
301-309-8504 x2121