RE: BIG files
Doug Smoot (rin0dss@bumed30.med.navy.mil)
Wed, 5 Jan 1994 09:54:38 -0400 (EDT)
I have had a similar problem with my Elite. I discovered that the problem
in my files was in the header. When I saved my files I got an error
message saying the there was a problem saving the file for some reason I
can not remember. The FCS file format only allows for a certain size
file. I ran my files through an FCS file reading program we created and it
determined that the data area was invalid (It has been a while but I think
that is because there are not enough digits in the header space to allow
for a very large data area. What I now do is pregate the data using
the listgate feature (under the options screen on the cytometer - useful if
you are gating out non-indo loaded cells if you are running an indo
experiment). This doesn't really help with the files you have already saved
though.
In message 3 Jan 94 10:13:00 EST,
"IMVS4::KWEBER" <KWEBER%IMVS4.decnet@uv1.im.med.umich.edu> writes:
> HELP!!
>
> We have saved a number of listmode files on the Coulter Elite that are
> in excess of 10 MBytes. Unfortunately, the Elite software does not
> recognize them as valid listmode files, and the WinList software cannot
> handle displaying all 1,000,000 data points in the files. We desparately
> need to analyze these files and would appreciate it if anyone who has run
> into this problem could let us know how to work around it.
>
> Thanks!
> Kris Weber
> University of Michigan
> (313)-747-3216
> fax: (313)-764-2566
> e-mail: kweber@uv1.im.med.umich.edu
{---------------------------------------------------------------------}
Douglas Smoot
Naval Medical Research Institute, Code O6
Bethesda, MD 20889-5607
(301) 295-1121