In message Fri, 20 Aug 1993 16:48:59 PST,
Mike_Lance@bdis.com (Mike Lance) writes:
> Subject: Time:4:38 PM
> OFFICE MEMO Ratio on FACS Vantage/FACStar+ Date:8/20/93
>
> The FACS Vantage and the FACStarPLUS are both highly adaptable. However,
> ratioing the delayed signals between two lasers is not possible on either
> system. The suggestion of using the FL2 signal cable to "trick" the
> instrument will result in signals from two events being ratio'ed, not two
> parameters from a single cell being ratio'ed which is probably what you
> want. This is because of the timing of when the signal is ratio'ed, which
> occurs right after the peak. Thus, the ratio is determined right after the
> the particle goes through the first laser (on an FL1/FL2 ratio), before it
> is even seen by the second laser! So if you "trick" the system this way,
> the ratio is actually the ratio of an FL1/"FL2" event that occurred at the
> same time as an actual "FL4" event.
>
> Angela Schultz
> Product Manager, FACS Vantage
{---------------------------------------------------------------------}
Douglas Smoot
Naval Medical Research Institute, Code O6
Bethesda, MD 20889-5607
(301) 295-1121