Hepatocytes

hilliard@zookeeper.zoo.uga.edu
Wed, 14 Apr 93 15:41:42 EST

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I have a user who's advisor just told her "find a protocol for DNA
analysis of liver cells in fish--we'll be collecting samples the first
week of May". Vindelov and Christensen's procedure (in Darzynkiewicz and
Crissman) seems too easy--does anyone have any caveats? How should they
process samples--freeze a section dry at -70 and needle aspirate in the
lab? Aspirate from fresh and rinse into buffer to freeze? How will they
avoid lots of RBC's? Any warnings will be helpful--we'll be working out
the kinks soon (hopefully!).

Steve
Steve Hilliard : "Be good, and you will be
Cell Analysis Facility, Univ. of GA : lonesome."
email: hilliard@zookeeper.zoo.uga.edu :
voice: (706)542-9474 : I brew the beer I drink!

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