I have a very basic question, and am hoping it is not too off topic for
this group. I am being asked to analyze by flow, mouse lymphocytes. They
will first require a density gradient procedure to isolate the
lymphoctes.
I will have very little volume of blood to use, and would like to have
an idea as to the minimum volume of whole blood I would need in order to
analyze for CD4, CD8 and CD19 (Ts and total Bs). I sense I can do this
in 2 tubes (experimental and control), so I will need enough for both
tubes.
Does any of you help me?
Please e-mail me privately at echeagaray@sri.org
Thanks in advance!
P. Echeagaray