E.coli - GFP- Posted FOR....

J.Paul Robinson (robinson@flowcyt.cyto.purdue.edu)
Wed, 23 Jul 1997 16:03:35

NOTE about postings: I am receiving many postings from colleagues who
review this list on our website. I try to post them but I think that if
people are interested enough to survey the web site, that in general they
should decide to accept the email messages as everyone else. Some are new
users who discover the EMAIL site and find it useful - these are the people
that I put messages up for - others give the argument that they get too
much email so they should be able to send messages, but not receive
everyone elses. Remember the postings are not automatic like many others -
that's why there is relatively little junk mail! My philosophy is that if
you have to read them all, you will be careful what you send!! Sorry this
diatribe has nothing to do with the following message which asks an
important question!!

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The following message is posted for Virginia Cox <cox@medicine.tamu.edu>.
Please reply to CYTOMETRY or to her address with a response.

>I have been trying to sort E. coli with the GFP gene inserted for a
>client. When we run the sorted bacteria through the machine to check for
>purity, we see little fluorescence & the light scatter has changed. We
>think we may be damaging the cells. Any experience or suggestions would
>be appreciated.
>
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Posted by
>
__________________________________________________________________
J.Paul Robinson, Ph.D., Professor of Immunopharmacology
Director, Purdue University Cytometry Laboratories
Purdue University Phone:(765)-4940757 FAX: (765)-4040517
EMAIL: robinson@flowcyt.cyto.purdue.edu WEB: http://www.cyto.purdue.edu