B cell markers
Dr William Smith (williams@ichr.uwa.edu.au)
Fri, 11 Jul 1997 17:27:05 +0800
I have been working with human PBMC and trying to separate populations.
I've found that the B cell markers CD19 and 20 are useful but that they
appear to be down regulated after incubation of the cells for 24 or 48
hours, so I can no longer distinguish them as a separate population.
Could anybody please suggest a marker that is more stable, preferably one
that might be generally available, conjugated to FITC?
I'm not a B cell expert as you might gather. I find that the markers CD19
and 20 aren't very bright- much less than CD3 or CD14 for example- is this
due to low antigen density, or do I have duff antibodies?
Help appreciated.
Cheerio, William
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Dr William Smith
Institute for Child Health Research
(Company Limited by Guarantee ACN 009 278 755)
Subiaco, Western Australia, 6008
PO Box 855 West Perth WA 6872
Ph 61 8 9340 8792/8388, Fax 61 8 9388 3414
email williams@ichr.uwa.edu.au
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