Nigericin contamination?
Wayne Green (Wayne.Green@genetics.utah.edu)
Thu, 10 Jul 1997 11:42:11 -0600
We have been running some pH studies using SNARF-1 on myocytes. The
investigator wants to use Nigericin to calibrate the pH measurments but we have
some concern about contaminating the sample lines and flow cell in the flow
cytometer. A very recent paper by P.H. Richmond & R.D. Vaughan-Jones (Pflugers
Arch - Eur J Physiol, No. 254, 1997- I have only provisional page numbers)
indicates that residual nigericin can strongly affect subsequent nigericin-free
samples in a superfusion flow chamber. They developed several extensive
cleaning procedures to remove the contaminating nigericin from their apparatus.
I have searched through the flow literature and have found references in
which nigericin is mentioned as a means of calibrating measurements, but have
found nothing addressing contamination of the instrument or cleaning nigericin
from tubing/flow cells. Does anyone have experience with this compound and is
contamination a problem in a flow cytometric application?
--
Wayne Green
wgreen@genetics.utah.edu