you can try abe schwartz's simply cellular beads. call Flow cytometry
standards corp. 800 227-8143. they're fairly easy to use and you can
extrapolate the number of Ab bound to a cell (average of a population
actually)
hope this helps
gary woo
>----------
>From: Ras Smit
>Sent: Wednesday, July 9, 1997 8:59 AM
>To: Cytometry Mailing List
>Subject: Semi-quantitation of CD4 receptors
>
>
>Hallo everybody
>
>I believe a similar question has been recently asked, but can anybody
>tell me how to determine the density of CD4 receptors on lymphocytes
>with flow cytometry. Any previous methods discussed on
>this mailing list or references will be greatly appreciated.
>
>Ras Smit
>Dr EJ Smit; Registrar in Virology, Medical University of Southern Africa
>PO Box 173, MEDUNSA, 0204, South Africa
>Phone +27 12 521-4113 Fax +27 12 521-5794
>