does anybody know a source for fluorescent beads with changing fluorescence
properties (such as excitation of emission spectrum) with the pH value. I
want to measure the pH value of the lysosomes of phagocyting macrophages. The
beads should have about 2 um diameter. I have beads which show variations,
but they are really not monodispers, the fluorescence distribution is
extremely broad. I would like to have a sharper distribution. All laser
wavelength from UV to HeNe could be used.
Thank you in advance
Best regards to all flow people
Wolfgang Beisker
Dr. Wolfgang Beisker
GSF - Durchflusszytometrie phone: (+49)(0)89 3187 2842
Ingolstaedter Landstr. 1 FAX : (+49)(0)89 3187 3349
85764 Neuherberg EMAIL: Beisker@gsf.de