We often do T-cell lymphocyte subset analysis after Cd34 collection.
The reason being that most of our allogeneic transplants are T-cell
depleted. We however use Campath 1 which often depletes other cell
lines as well. In our experience there is a huge drop in the T-cell
population but we have not seen the huge increase in CD19+ cells that
you have seen. We also haven't experienced the CD3+/CD19+ population
you describe.
I hope this has been helpful. I would be interested to hear what
others have experienced.
Good luck,
Glenda Davison
Dept.Haematology Groote Schuur Hospital
University of Cape Town
South Africa
email: gmd@samiot.uct.ac.za