In my laboratory work, I attempt to double stain the cells with Brdu (for
proliferation assay) and a cell surface marker. The cells I am working
with are the cell mixture of intestine epithelial cells and T cells. I
don't know if anybody has tried to do the similar things. It seems a
little hard to do since the anti-Brdu Ab has to enter the nucleus while
the other Ab has to bind to the cell surface moleculels. I would
appreciate any input. My email address is wangx01@doc.mssm.edu
Tian Wang
Clinical Immunology
Mt. Sinai School of Medicine
Tel:(212)-824-7466