Erythroleukemia in rodents?

James Weaver 301-594-5879 FAX 301-594-3037 (WEAVER@cder.fda.gov)
Wed, 02 Jul 1997 15:58:26 -0400 (EDT)

Group:
In a mouse model system that we are evaluating, it is
claimed that one of the "common" results is production of
erythroleukemia (aka AML-M6). We would like to sort these cells from
treated mice and grow them in culture to provide enough cells for
evaluation of each clone by genetic methods. My review of the few
sources of information that I could locate suggests that there is
less than total agreement on the diagnostic criteria.

My questions for the assembled experts:

(1) Is there a common set of surface markers that could reasonably
be considered to define erythroleukemias. {I cannot use glycophorin
A as that is an internal marker}.

(2) If so, are there sources of these antibodies that can be used to
detect mouse cells?

Thanks for any and all responses,

-Jim Weaver

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* James L. Weaver Ph.D. *
* Division of Applied Pharmacology Research *
* Office of Testing & Research *
* CDER MOD-1, FDA *
* 8301 Muirkirk Rd, Laurel MD 20708 *
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* Phone: 301-594-5879 *
* Fax: 301-594-3037 *
* Email:WEAVER@CDER.FDA.GOV *
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