Monocyte sorting

Stacie Anderson (staciea@nhgri.nih.gov)
Fri, 27 Jun 1997 10:30:22 -0400

We are interested in sorting viable human monocytes (CD14+) to be used in
functional cytokine production assays. We have done the experiment once
with poor monocyte viability. The monocytes were sorted, placed into
culture for 24 hours and then stimulated with LPS and monensin. The
combination of these events killed the monocytes - any suggestions? Thanks
in advance for any suggestions. Stacie Anderson

Stacie Anderson
Laboratory of Gene Transfer, Hematopoeisis Section
NCHGR, NIH
Room 2C12
49 Convent Drive
Bethesda, MD 20892-4442