Re: Distance between sort streams
Joseph Webster (J.Webster@centenary.usyd.edu.AU)
Fri, 13 Sep 1996 10:37:20 +1100
At 08:25 AM 12/09/96 +0300, Yash Pal Agrawal wrote:
>Hi everybody,
> I request some advice regarding sorting.
>Question: We use a FACStar for sorting. However, inspite of my best
>efforts I am not able to get the Left and Right Streams to separate much
>from the center stream. As a result the sorted cells miss the collection
>tubes. (The left and rt. stream deflection controls are turned to max).
>
>- Does the angle between the 2 charged metal plates (which is adjustable)
>have anything to do with it ?.
We had a similar problem with the FACStar Plus and found that the
deflection plates were upside down!
Yes, the angle does make some difference, but the longer part of the
plates must be UP towards the nozzle.
We run with the left & right controls at Max and the streams are about
centre of each collection tube. (cannot make it go near the outer edge
of collection tubes)
Good Luck, Joseph.
Joseph Webster (O.I.C. Flow Cytometry)
Centenary Institute of cancer Medicine & Cell Biology
Locked Bag No.6, Newtown, NSW 2042, AUSTRALIA.
Ph: 61-2-9565-6110 Fax: 61-2-9565-6101