Propidium Iodide and Calcium

Michael J. Herron (herro001@maroon.tc.umn.edu)
Thu, 23 Nov 95 10:51:27 -0600

Does anyone know anything about the potential effects of calcium and magnesium
ions on propidium iodide/DNA staining? I have made up the PI in DPBS that
either does or does not contain calcium and magnesium and I get rather different
histograms.

It could be something else in my experiment...but I thought I would check and
see if this is a well known phenomenom before continuing.

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/ Michael J. Herron University of MN, Dept. of Dermatology /
/ herro001@maroon.tc.umn.edu /
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