Re: Visual detection of CY-5

GREG VAUGHN MARTIN (gmartin@welchlink.welch.jhu.edu)
Sun, 19 Nov 1995 11:42:43 -0500 (EST)

Hey Daryl --
I have had good results visualizing Cy5 with a 100W HBO lamp on a
standard epi-fluor. 'scope using Zeiss' "00" filterset (excitation:
BP 530-585, beamsplitter: FT 600, emission: LP615) which is a filterset
designed for visualizing Texas Red. However, you can't see cy5
fluorescence by eye. If you have a CCD camera you can use it to capture
the Cy5 image as long as the CCD has decent spectral response above
~650nm. Many CCD cameras have an IR cut off filter so that they will
match human vision. For the camera I use (Dage 72) it was a trivial
matter to remove the IR cut off filter.
Good Luck,

Greg Martin
Dept. Cell Biology and Anatomy
Johns Hopkins School of Medicine

On Fri, 17 Nov 1995, Daryl Webb wrote:

> Hi all, does anyone have experience with CY-5 on a "standard" HBO 50 lamp
> based Fl microscope. I've heard that it can be difficult to see (as opposed
> to detect with pmt's), the local sales droids have never sold the appropriate
> filter block so wont commit themselves. Dont particularly wish to fork out
> for the filter block if I wont be able see my results...
>
> Anyone with advise/experience.
>
> ta muchly
> --
>
> Daryl Webb (dwebb@waite.adelaide.edu.au)
> Dept. of Plant Science, Waite Institute
> University of Adelaide, Glen Osmond S.A. 5064
> Australia. Voice:61_8 303 7426 Fax:61_8 303 7102
>
>
>
>