detection of mRNA by flow cytometry
Dr. Howard Petrie (h-petrie@ski.mskcc.org)
Wed, 15 Nov 1995 11:42:10 -0400
Dear fellow cytometrists: I am interested in attempting to detect mRNA on
a single cell basis by in situ staining and flow cytometry. I am aware of
a few papers in the literature (globin message), but they are a little
dates now. I am wondering if anyone could share their experience regarding
similar applications. Are biotinylated riboprobes most likely to be
useful? Which biotinylated nucleotides have been used? How can signal
intensity/ background ratios be maximized? I will repost a summary in the
case of multiple responses. Thanks in advance. HTP