Re: gfp ser mutant
RCherv (RCherv@mail-sh.lsumc.edu)
Tue, 07 Nov 95 22:48:18 cst
Rochelle,
We have been using this same gfp mutant with some success recently.
We started this work on a FACS Vantage with a standard FITC setup
(488nm excitation; 530/30 bp filter) and have seen good fluorescence
from transiently transfected murine cells and from cells infected with
a mutant gfp-encoding retrovirus. We are now using an improved
version of this setup, in which the 530/30 bp filter is replaced with
a 510/20 (Omega Optical). This has the dual advantage of cutting down
background from autofluorescence and increasing the signal from the
gfp. With either setup, the mutant gfp is better/brighter than the
original excited by UV in our hands. If you were able to detect
expression of the original gfp, I don't think you'll have any problems
with the mutant. If you do, let me know.
Good Luck
Rob Chervenak