Re: HIV/flow analysis

Lt Col Bill Ward, Wilford Hall Med Cen/PSLCI, (ward@WHMC-LAFB.AF.MIL)
Mon, 6 Nov 1995 09:35:44 -0500

The current guidelines for CD4 enumeration as published by the CDC are:

Centers for Disease Control and Prevention. (1994) 1994 revised guidelines
for the performance of CD4+ T-cell determinations in persons with human
immunodeficiency virus (HIV) infection. MMWR 43(RR-3):1-21, March 4, 1994.

We don't have a procedure that describes the use of this panel per se, but do
have multiple procedures concerning two- and three-color immunophenotyping in
general. I'd be happy to share these with you if you are interested.

Bill Ward

---------------------------------------------------------
William W. Ward, Ph.D. Phone: (210)
670-6838
Immunology & Molecular Diagnostics Branch FAX: (210) 670-7484
Department of Pathology email:
ward@whmc-lafb.af.mil
Wilford Hall Medical Center/PSLCI
2200 Bergquist Drive, Suite 1
Lackland AFB, TX 78236-5300
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>Date: Fri, 03 Nov 1995 13:56:55 -0500 (EST)
>From: Frederic Preffer <preffer@helix.mgh.harvard.edu>
>Subject: HIV/flow analysis
>To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
>
>Will someone kindly provide ref. of most RECENT guidlines for enumerating
>PBL of HIV+ patients. Thanks.
>
>PS. If you happen to have protocol in a format that conveniently can be sent
>with your message text (a few paragraphs from your lab manual that details
>reference) that would be appreciated too.
>`````````````````````````````````````````````````
> Frederic I. Preffer
>preffer@helix.mgh.harvard.edu
>Department of Pathology- Cox5
>100 Blossom St
>Massachusetts General Hospital
>Boston MA 02114
> (617) 726-7481 {fax x2365}
>~~~~~~~~~~~~~~~~~~~~~~~~~~~