We have had the same problem here a couple of times using a FACScan
(Cellquest 1.2.1), several users have aquired data in a series, and 1 or 2
of the samples are showing completely random data in the analysis (although
during the acquisition data looks fine..). So MaryAnn is not alone !!!!
I contacted BDIS in France, as well as in the US, concerning this problem,
but they haven't replied yet... I think this must be a problem related to
some memory screw up, because for us it only happened if people were
acquiring more than 100 000 cells
I hope the BDIS guys are on the list !!! And do something to solve this
soon.... I don't want to go back to our old HP LYSIS...
Matthias
______________________________________________________________
Matthias Haury _/_/_/ _/_/_/ _/_/_/
Flowcytometry - Immunology _/ _/ _/ _/ _/
Institut Pasteur Paris _/ _/_/_/ _/_/_/
Email: mhaury@pasteur.fr _/ _/ _/
Tel: 33 (1) 40 61 31 29 _/_/_/ _/ _/
Fax: 33 (1) 45 68 86 39 INSTITUT PASTEUR PARIS
______________________________________________________________
At 9:37 3/11/95, mdemaria@warren.med.harvard.edu wrote:
>Hello everyone,
>
> I know there have been recent discussions on certain Cellquest bugs and I
>was wondering if anyone has ever experienced the following:
>
> We were aquiring a series of samples using Cellquest on a FACS Vantage. It
>took about 20 minutes to run the entire set. During aquisition, all
>samples had
>appropriate staining patterns when looking at FL4 vs FL5. However, when we
>went
>to analyze the samples, 3 out of the 15 samples had randomly changed all the
>parameters. FL4 and FL5 staining was lost, FSC and SSC even were lost. We
>discovered that FL4 was now in FL2 and FL5 was in FL4 and we could not
>find FSC
>or SSC. We can't understand this because all samples were run at the same time
>and it was not done in 3 consecutive samples but rather at random.
>
> This happened 2 days in a row.
>
>Thanks everyone,
>
>MaryAnn DeMaria
>Harvard Medical School
>New England Regional Primate Research Center
>508-624-8029