Indo-1 in CHO cells -Reply
mohedley@spiff.pmh.toronto.on.ca
Sat, 04 Nov 1995 08:51:53 -0500
Along with several other cell lines, CHO cells can express very high levels
of p-glycoprotein, and this actively pumps indo-1 out of the cells. The
solution is to load in the presence of a pgp inhibitor such as 10-30 uM
verapamil. We published an account of this problem in Cytometry last year.
David Hedley
Princess Margaret Hospital
Toronto