Detection of Cy5

colette charland (ccharlan@moose.uvm.edu)
Thu, 2 Nov 1995 12:55:13 -0500

Messages sorted by: [ date ][ thread ][ subject ][ author ]
Next message: mohedley@spiff.pmh.toronto.on.ca: "reference managment software? -Reply"
Previous message: Rochelle A. Diamond: "gfp ser mutant"

Greetings from the U. of Vt. I've just received a call from an investigator
who is going to label platelets with a FITC conjugated marker and an
activation marker linked to Cy5. They are using a kit from Amersham LIFE
SCIENCE called FluoroLink-Ab Cy5 Labeling Kit.The detection of plts. is no
problem, but I've never worked with Cy5. Does anyone have experience with
this kit and/or running such specimens on the flow. Any problems that I
need to know of in advance, i.e. quenching, compensation, coating of sample
tubing with the dye--you know the usual problems. Suggestions regarding
filter set up or any other input you have will be appreciated
Thanks in advance,

Colette Charland
ccharlan@moose.uvm.edu

Next message: mohedley@spiff.pmh.toronto.on.ca: "reference managment software? -Reply"
Previous message: Rochelle A. Diamond: "gfp ser mutant"