Re[4]: Query regarding cell cycles

Dennis_Young@CIS.ucsd.edu
Tue, 24 Oct 1995 12:12:00 -0700

I apologize for seeming to slam ModFit...

> What I was saying is that I've not used the software in question (ModFit), so
> didn't say anything other than I don't use it.
(I don't *have* it.)

> I was not comparing the two software packages. Sorry that was not clear. I was
> merely saying that Multicycle is better than weighing cut-up graphs (no extra
> software involved).
>
>
> Dennis
> > The cheapest is to cut out the peaks and weigh them!
> > We use Phoenix Flow Systems' Multicycle, which does a *much* more
> > sophisticated
> > job.
> >
> > (619) 453-5095
> >
> >
> > Verity Software House has a DNA analysis program; don't use it.
> >
> >
> > _/_/_/ _/_/_/_/ _/ _/
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> > _/ _/ _/ _/
> > _/ _/ _/ _/ _/
> > _/ _/_/ * _/_/_/ * _ /*
> >
> > ****************************************************************************
> > * Dennis J. Young Voice : (619) 822-0407
> > * Flow Cytometry Core Facility FAX : (619) 822-0412
> > * University of California, San Diego USA e-mail: djyoung@ucsd.edu
> > ****************************************************************************
> >
> >
> > ______________________________ Reply Separator _____________________________
> > Subject: Query regarding cell cycles
> > Author: dgalbrai@ag.Arizona.EDU at @UCSD
> > Date: 10/11/95 4:31 PM
> >
> >
> > I have a colleague who is getting cell cycle data and histograms from (and I
> > quote) "a FACSscan 8383 with Consort 32 software, and Cellfit version
> > 2.01.2". It apparently produces HP formatted floppies with the data in FCS
> > 1.0 file
> > format. They have some unusual cells that arrest in early S-phase and the
> > cell cycle software lumps this S-phase stuff in with the G1 fraction. Not
> > good.
> >
> > They wondered whether there were any programs available to convert the HP
> > FCS 1.0 files into something that can be read by other cell cycle programs?
> > -- also as to which cell cycle programs might be most appropriate for this
> > type of application. Any suggestions as to what might be best/cheapest etc.
> >
> >
> > Thanks in anticipation!
> >
> >
> > David Galbraith
> >
> >
> > ==============
> > David Galbraith
> > Professor of Plant Sciences
> > University of Arizona
> > 303 Forbes Building
> > Tucson AZ 85721
> > Phone (520) 621-9153
> > FAX (520) 621-7186

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*****************************************************************************
* Dennis J. Young Voice : (619) 822-0407 *
* Flow Cytometry Core Facility FAX : (619) 822-0412 *
* University of California, San Diego USA e-mail: djyoung@ucsd.edu *
*****************************************************************************