Agarose microdrops

Mrs L M Dash (lmd2335@ggr.co.uk)
18 Oct 95 16:20:00 BST

I would like to select transfected cells on the basis of their secreted
proteins. I have a paper (Kenney et al.) which describes the use of agarose
microdrops and cell sorting to separate hybridomas producing appropriate
antibodies.
I would be grateful if anyone out there with experience with this technique
has answers to the following questions:-

1) Do I need specialised eqipment to make reasonable microdrops? How much
does it cost?
2) How straight forward is the method of preparation and sorting with
microdrops? Are there any technical problems I can expect and do you have
any tips for trouble shooting?

Thanks in advance for your help,
Laura Dash (Glaxo Wellcome).