Re: Anti-Platelet Abs and Reticulated Platelets

Gregor Rothe (Gregor.Rothe@klinik.uni-regensburg.de)
Fri, 13 Oct 1995 16:51:07 +0100

> Date: Thu, 12 Oct 1995 04:54:24 GMT
> From: hugh@.cei.net
> To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
> Subject: Anti-Platelet Abs and Reticulated Platelets

> Does anyone have a good Anti-platelet IgG assay they are using?
> How about Reticulated platelets. We have been using protocols
> published by Kinder te al Arch Pathol Lab Med v117 1993 for
> Reticulated platelets and are not entirely satisfied with
> the very dim fluorescence. Any takers?
> Hugh Johnson
> Flow Cytometry
> St Vincent Infirmary Med Center
> 2 St Vincent Circle
> Little Rock AR 72205-5499
> hugh@cei.net
>

Fluorescence resonance energy transfer can be used for the
simultaneous quantification and epitope-specific characterization
of anti-platelet antibodies. A manuscript giving details of the
method is currently in press in the Journal of Immunological
Methods.
Preprints and further information on request.

Gregor Rothe

***********************************************************
Institute for Clinical Chemistry and Laboratory Medicine
University of Regensburg
D-93042 Regensburg, F.R. Germany

Tel. +49 (941) 944-6208, or -6233
Fax +49 (941) 944-6202

Internet: Gregor.Rothe@KLINIK.UNI-REGENSBURG.DE
***********************************************************