Re: Right angle scatter with UV laser line?

Ray Hicks (rh208@cus.cam.ac.uk)
Thu, 12 Oct 1995 10:17:15 +0100 (BST)

Hi Ray,
this may be a rude question, but did you take out the 480 band pass
filter from in front of the SSC PMT?. If you did, you may be able to put
more light in that direction by replacing the coverslip that diverts
light in the SSC direction with a long pass dichroic.

Ray

> Do you not get right angle light scatter (or very little of it) when
> using the UV line? I was just setting up for an experiment in which we
> will be using UV on our FACS 440. For alignment, I was using some old
> Polyscience beads
> (about 5 micron dia.) that still fluoresce and I didn't see much side
> scatter.
> Should I? Will it be different with cells, i.e., will I see SS with cells
> even though I don't see it with these beads? If not, is it a property of