We detect MPO using anti-MPO-FITC from DAKO, Carpenteria, CA with the
fixation/permeabilization reagent from Pinnacle Biosystems, Burlingame,
CA. We use it on blood and bone marrow and the antibody and reagent
seems to work fine.
We use a number of other antibodies from DAKO with this
fixation/permeabilization reagent and they
work fine (anti-CD3, anti-kappa, anti-lambda, anti-PCNA, etc.)
We however almost always start with ficolled cells from blood or bone
marrow. Some whole blood studies are done after rbc lysis.