This one has me scratching my head--we're using DAPI to stain for DNA
content, and our first sample always has a lower fluorescence than
the subsequent samples. We thought at first it was staining
variability, but out of curiosity we re-ran the first tube at the end
of the experiment, and it had gotten brighter, so that all cells had
similar DNA content (as we were expecting). This has been consistent
through the last 3 visits.
Is this some sort of tubing/sheath equilibrium effect? I hadn't
heard of this with DAPI, but we haven't done that much with it. has
anyone seen this before?
Steve
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Steve G. Hilliard Life's too short
Cell Analysis Facility to drink bad beer!
University of Georgia