plant chromosomome sorting
Sergio Lucretti (lucretti@casaccia.enea.it)
Mon, 18 Sep 1995 12:56:08 +0100
Dear Julie, we have done quite a bit of work on plant chromosome analysis
and sorting with a FACStar Plus. Our instrument, for this purpose, did not
need any complete modification, the only change from standard configuration
beeing the "MACROSORT" option which we have found useful always. Our
chromosome experience involves chromosome isolation mainly from legume
species (field bean, pea) stained with PI, DAPI or DAPI/MI. In
monoparametric analysis, DAPI gives us the best resolution, providing
chromosome DNA contents differ of about 2.5% between them (according to our
measurement CV). DAPI/MI was useful only for discriminating chromosomes in
some reconstructed karyotypes of field bean, since the standard katyotype
has almost the same A-T/C-G base content. In our experience, we have found
quite useful to use "pulse processing" to encrease discrimination between
chromosomes, debries and small chromosome doublets (e.g. combining DNA-FPA
versus DNA-FPW). I hope you could get some help and informations in our
works on TAG 85:665-672 (1993), Crit Rew PS 13:275-309 (1994) and TAG
90:797-802 (1995). I don't know which "green" chromosomes you are looking
for, but I hope you could make some use of our comments.
Please, let me know if you would need some more informations. Sergio.
Sergio Lucretti
ENEA C.R. Casaccia
Biotechnology & Agrigulture Sector
C.P. 2400 - 00100 Rome (Italy)
phone ++/6-30483191
fax ++/6-30484808