Aneuploidy and Polyploidy -Reply
mohedley@spiff.pmh.toronto.on.ca
Mon, 11 Sep 1995 17:09:45 -0500
Tetraploidisation is probably quite common in long term culture. For
example, nearly all the CCRF-CEM lines in use in North American labs
seem to be tetraploid. In Sydney we used diploid cells, and cleaned out
the tetraploids periodically by centrifugal elutration. I guess most labs don't
track their cell lines with DNA flow cytoemtry.
Don't know if this makes the experiments any better, though!
David Hedley
Ontario Cancer Institute
Toronto