> Hello fellow cytometrists,
>
> One of our researchers wants to "measure apoptosis in cell
> suspensions made from solid tumours fixed in 70% ethanol (or other
> fixatives as apprpriate)."
>
> Can anyone one point us in the right direction?
>
> Thanking you for your time and trouble ....
>
I presume he means an end-labelling technique with TdT and FITC labelled
d-UTP. We have been doing this for a while and it seems fairly reliable.
Try Bromidge et al, Cytometry 20: 257-260, 1995.
If you wish I have a working protocol which I can post.
Eric Miller, ICRF Medical Oncology Unit, Edinburgh UK