Bovine sperm sorting

PAUL@flowcyt.cyto.purdue.edu
Fri, 25 Aug 1995 09:31:35

Roger Burger asked about sorting bovine sperm. We have a publication
on this - it was done by magnetic beads and confirmed by flow
cytometry sorting and analysis. The details are in the papers below.

Paul

Jones, P.P., Peter, A.T. and Robinson, J.Paul - Monoclonal antibody
12/49 separation of X and Y chromosome-bearing bovine spermatozoa
using immunomagnetic beads and flow cytometry. Proc.Conf.Res.Workers
Animal Dis. 72:30-Abstract 173, 1991.(Abstract)

Peter, A.T., Jones, P.P. and Robinson, J.Paul - Fractionation of
bovine spermatozoa for sex selection: a rapid immunomagnetic
technique to remove spermatozoa that contain the H-Y antigen.
Theriogenology 40:1177-1185, 1993.

J.Paul Robinson, Purdue University Cytometry Labs
robinson@flowcyt.cyto.purdue.edu PH:317-494 6449 FAX:317-494 0517
web http://www.cyto.purdue.edu