flow crossmatch

LUIDER@Gonzo.FhHosp.Ab.Ca
Thu, 17 Aug 1995 11:46:55 -0600 (MDT)

We need advice/information on what's happening out in the world as far as
using flow cytometry crossmatch methods vs the cytotoxicity methods (CDC).

Some of our questions are:

1. Do you screen and/or identify PRA by flow?
-do you still use/report CDC results as well?
-what do you do when the flow result is positive and CDC is negative?
_do you use pooled random donor cells to screen? Fresh/frozen?
_advantages/disadvantages?

2. Do you do flow crossmatching prospectively for renal(or other organs)
tranplants for living related or cadaver donors?
_do you still use/report CDC results as well?
-what results are used if the results differ?
-what methodology is used for flow crossmatching? ficolled cells/whole
blood lysis? T and B?

Thanks in advance everyone!

Joanne Luider
Foothills Hospital
Calgary, Alberta
phone (403)670-4765
fax (403)270-4135