> We have been trying to determine percentage of apoptotic cells using PI
> staining and analysis of DNA histograms with Modfit LT. The program works
> very nicely, but occasionally we get a histogram in which a substantial number
> of cells appearing in the G0/G1 and the S peaks are considered by Modfit as
> apoptotic cells. Verity does not provide any "explanation" or suggestions
> regarding how to get around that. Did anyone out there encounter this same
> problem and how was it resolved? Thanx for any info.
>
> Edward F. Srour, Ph.D.
>
>
Could you post a little more info on this? I have not managed to successfully
identify apoptosis on a DNA histogram on a Facscan. The samples are FNAs of
xenografted human breast tumour cell lines.
Eric Miller, ICRF Medical Oncology Unit, Edinburgh UK