FDG staining for b-gal

Rochelle A. Diamond (diamond@cco.caltech.edu)
Tue, 1 Aug 1995 18:07:14 -0700 (PDT)

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Cyto Listers-
I am about to embark on trying to stain transgenic mouse lymphocytes
carrying a b-galactocidase reporter gene with FDG for sorting. I could use
advice, protocols, and/or encouragement from people who have used this
successfully in mouse cells especially primary transgenic immune cells.

I am purchasing the FDG from Molecular Probes. Any help would be useful.

Thanks in advance,
Rochelle Diamond
California Institute of Technology
Division of Biology
Flow Cytometry/Cell Sorting Facility
diamond@cco.caltech.edu

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