surfs up, gotta'go.
cp
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Brett Lindenbach wrote:
>Hello. I'd like to look at expression of a surface protein on mouse
>macrophage-
>like cell lines by flow cytometry. I'm worried about decorating them with our
>mouse mAb, which could be bound by FcR's on these cells. Two options I thought
>of were 1) absorb mouse myeloma proteins to the FcR's, and FITC-label by mAb;
>2) cleave the mAb into Fab or F(ab')2 fragments which could be
>FITC-conjugated.
>What have other people done in these circumstances? What works and what
doesn't?