DAPI

Alice L. Givan (Alice.L.Givan@Dartmouth.EDU)
26 Jul 95 15:46:57 EDT

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We have a scientist who uses DAPI to discriminate between live and dead cells
(as with PI). However, Molecular Probes say that DAPI is a cell permeant DNA
stain (like the Hoechst dyes) and will stain living cells. Is this just a
kinetics difference so that DAPI enters living cells just a bit slower than it
enters dead cells? Has anyone used DAPI as an alternative to the Hoechst dyes
to stain living cells for ploidy? We want to be able to sort living cells on
the basis of their DNA content -- for further culture. Any suggestions?
Thanks!

Alice Givan
Englert Cell Analysis Laboratory
Dartmouth Medical School
Lebanon, NH 03756, USA
tel: 603-650-7907
fax: 603-650-6130

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