Re: surface staining macrophages

S.P. Monard (spm1007@cus.cam.ac.uk)
Wed, 26 Jul 1995 09:44:13 +0100 (BST)

Hi
When staining human macrophages we would pre-incubate the cells with 2
percent human serum for 15 mins at room temp before the primary antibody
step and keep 2 percent serum in all the wash buffers thoughout staining,
this worked very well and is much less trouble than digesting and
conjugating antibodies. A common problem when staining mouse cells is
cross reactivity between mouse and rat Igs, make sure your anti-rat
secondary antibody is absorbed against mouse Igs (I assume your primary
ABs are rat).
Best of luck

Simon Monard
Cambridge
UK