______________________________ Reply Separator _________________________________
Subject: Re: Antibromodeoxyuridine MoAb
Author: Robin.Allen@WPO4.CL.HealthWaikato.HWL.synet.net.nz at InternetMail
Date: 7/14/95 5:39 PM
Many thanks to all who replied to my request for a source of the BU-1
clone.
In reply to those who asked why the BU-1 clone in particular.
The commonly used antibromodeoxyuridine antibodies react only with
single stranded DNA which requires pretreatment with HCl for cleavage.
The BU-1 clone avoids denaturation of the DNA due to DNase activity.
This leaves cell morphology as well as surface or cytoplasmic markers
intact.
Robin Allen
Haematology Dept
Waikato Hospital
Hamilton
New Zealand
E-mail Robin.Allen@wpo4.cl.healthwaikato.hwl.synet.net.nz
Fax 64 7 8398759