Antibody Saturation
Edgar Milford (emilford@bustoff.bwh.harvard.edu)
Wed, 12 Jul 1995 10:08:38 -0500
In response to Mark Rehse's comments, it may be that some of the effect is
indeed due to quenching. Despite the fact that supersaturating amounts of
antibody are being used, it is possible that there is increased amount of
the antibody conjugate taken up by the particular cells being used. Without
some independent measure of SPECIFIC uptake at that concentration of
antibody it is difficult to determine whether this is the case or not. It
could be that a significant amount of the total fluorescence signal is
nonspecific binding and that this continues to increase until there is
quenching.
Edgar Milford
Edgar L. Milford, M.D.
Brigham and Women's Hospital
Tissue Typing Laboratory
75 Francis Street, Boston, MA, 02115
Tel: 617 - 732-5872
Fax: 617 - 566-6176