Sorting Hybridoma Cells

Neal Benson (nbenson@nervm.nerdc.ufl.edu)
Fri, 30 Jun 95 17:22:06 edt

One of my lab's users has several hybridoma cell lines that they would like
to re-clone to improve antibody production. It was suggested that they use
cell sorting to select the "high-expressers". They propose to label the
cells with a FITC-labeled anti-mouse IgG (subclass specific), then sort
those in the upper tail of the resulting fluorescence histogram.

In my limited knowledge of this area, I seem to have the impression that
expression of antibody on the cell surface does not necessarily correspond
to the quantity of antibody secreted from a hybridoma cell. I'd be very
interested to obtain opinions as to whether sorting of these cells based on
high surface expression is a reasonable thing to do for improvement of
antibody production.

Thanks in advance,
Neal
=======================================================================
Neal A. Benson Tel: (904) 392-0008
University of Florida Fax: (904) 392-4693
Department of Pathology Email: nbenson@nervm.nerdc.ufl.edu
Box 100275
Gainesville, FL 32610-0275 USA