We have used B-B4 on 4 cases involving plasma cell disorders. In
addition we tried known CLL and NHL to check specificity of B cell
stage.
To date no discrepancies have been observed where B-B4 was positive,
and no morphological evidence of plasma cells, or plasmacytoid
lymphocytes.
Plasma cell disorders studied so far show the following:
CD45 CD3 CD19 CD20 CD22 CD56 CD38 B-B4 IgG IgM IgA
I Marrow 21% 8% 0% 77%
I scalp lesion 0% 1% 0% 1% 94% 38% 72% 0% 4%
II LNBX 83% 0% 1% 0% 100 64% + + -
III Marrow 63% 1% 0% 2% 97 40 + +
In these cases cells were labelled with B-B4 and once fixed and
permeableized( Fix and Perm), 2 colour analysis was done for
cytoplasmic expression of heavy and light chains.
The B-B4 antibody was available through Immuno Quality Products.
One of the cases showed a dimmer expression of B-B4. This may be our
in-house problem where using the fix and perm reagent, I lost some of
the antigen.
I hope these comments may be of some use to you.
Paul.
Address:
Paul Harris
Flow Cytometry Specialist
Dept of Laboratory Medicine and Pathology
St. Joseph's Health Centre
268 Grosvenor St.
London, Ont.Canada N6A 4V2
P (519)646 6000 ext 5918
F (519)646 6088
E paulh@stj.stjosephs.london.on.ca
>>> Birger Christensson <BICH@patd01.hs.sll.se> 6/14/95, 03:10am >>>
Hi
One of the more recent "markers" proposed for plasma cells in
immunohistochemistry has been the BB4 antibody.
Has anyone information of the corresponding antigen, its
distribution?=
Is the gene cloned and published?
Has anyone been successful using it in FACS-analysis? Would you
detect 100% of myeloma cells???
Birger
-------------------------------------------------------------------------
Birger Christensson, MD, PhD
Dept. of Pathology, F49
Huddinge University Hospital,
S-14186 Huddinge,
SWEDEN,
Tel +46-8-7461000
Fax +46-8-7795520
bich@PATD01.HS.SLL.SE