Re: Transformation of scatter and FL signals

Dennis_Young@CIS.ucsd.edu
Wed, 31 May 1995 10:47:00 -0700

HP Paint-a-gate PLUS on the HP can generate transformed side scatter files, but
it is pre-programmed (ie, you don't control the transformation) and only side
scatter.
I'm sure you can transform data with WinList, because you can use ANY formula on
ANY parmeter (and combination thereof). I don't have it, but I've SEEN it :(

_/_/_/ _/_/_/_/ _/ _/
_/ _/ _/ _/ _/
_/ _/ _/ _/
_/ _/ _/ _/ _/
_/ _/_/ * _/_/_/ * _ /*

*****************************************************************************
* Dennis J. Young Voice : (619) 543-3928 *
* Flow Cytometry Core Facility FAX : (619) 543-7487 *
* University of California, San Diego USA e-mail: djyoung@ucsd.edu *
*****************************************************************************

To all of you,

As usual, I start by posting my problems to this list. Does anybody
know of a (commercial or non-commercial) software package that allows
analysis of transformed scatter or FL signals?
I know that Martz's MFI and Trotter's WinMDI supports the generation of
ASCII-based files, but the data files thereby reach sizes not easily
handled by computers, I can lay hands on. Also, I know that B-D
previously marketed Chronys, which apparently did the job, but it is
running on HP 310 (Pascal 3.1), and our old 310 hasn't got the speed and
RAM to master our sample files.
Is it possible to generate list mode FCS files with transformed data
included as new parameters?

Bjarne

_________________________________________________________________

Bjarne K. Moeller, MD
Dept. of Clin. Immunology Voice: +45 8949 5304
University Hospital of Aarhus Fax: +45 8949 6007
Skejby Sygehus
DK-8200 Aarhus, Denmark E-mail: bkm@biobase.dk

_________________________________________________________________

>-- Saved internet headers (useful for debugging)
>Received: from flowcyt.cyto.purdue.edu by mail.ucsd.edu; id LAA08000 sendmail 8
>Received: by flowcyt.cyto.purdue.edu (950215.SGI.8.6.10/930416.SGI.AUTO) for cy
>Received: from biobase.dk by flowcyt.cyto.purdue.edu via SMTP (950215.SGI.8.6.1
>Received: by biobase.dk; id AA22603; Wed, 31 May 1995 14:12:46 +0200
>Date: Wed, 31 May 1995 14:12:44 +0200 (MET DST)
>From: Bjarne Moeller <bkm@biobase.dk>
>Subject: Transformation of scatter and FL signals
>To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
>Message-Id: <Pine.3.89.9505311351.A21033-0100000@biobase.dk>
>Mime-Version: 1.0
>Content-Type: TEXT/PLAIN; charset=US-ASCII