Response to CD34 and apopto
Edward Srour (edward_srour@iucc.iupui.edu)
Tue, 30 May 1995 10:06:44 -0500
Steven Gore was asking for suggestions to label fixed cells with CD34. Try to
adopt the methodology we had used previously to study cell cycle status of
cultured CD34+ cells. You can find this in J. Immunol 148:815-20, 1992 or in
Exp Hematology 22:1264-72, 1994. Fixation of apoptotic cells with methanol
washes away the small size DNA fragments you are trying to label. Stick with
formaldehyde it works better.