Ray
On Thu, 25 May 1995, STEVEN DAVID GORE wrote:
>
> We have been trying to adapt the TUNEL assay to identify apoptosis among
> normal human CD34+ cells, double labeled with PE-labeled CD34 antibody.
> While this procedure works well for CD34-expressing cell lines, such as
> TF-1 or KG1a (the CD34 labeling performed before formaldehyde fixation,
> then followed by MeOH or EtOH etc), among isolated bone marrow CD34+
> cells, CD34 appears undetectable after the alcohol second fixation step
> (either alcohol). Does anyone have any experience or suggestions?
>
>
>
> Steve Gore
> Johns Hopkins Oncology Center
> Oncology 2-109
> 600 N. Wolfe St.
> Balto, MD 21287-8963
> sdgore@welchlink.welch.jhu.edu
> 410-955-8781
> FAX 410-614-1005
>