Hoechst 33342 emission in apoptotic cells

jau@imatge.sct.ub.es
Thu, 25 May 1995 19:01:04

Hi all! Can anybody help us?

We are trying to detect apoptosis in Jurkat cells, with Ho33342.
We see a shift in Hoechst emission to longer wavelenghths in apoptotic cells
respect to non-apoptotic ones. Can anybody explain?

Thanks,

Jaume Comas
Unitat de Citometria de Flux
Serveis Cientifico-Tecnics.
Universitat de Barcelona, Spain.
E-mail jaume@giga.sct.ub.es